800.227.0627

Bio-Synthesis offers DyLight™ 650 fluorescent dye oligonucleotide labeling services.  DyLight™ 650 is a far-red fluiorescence dye that are comparable to Alexa 647 and Cy5 dye for flourescent applications.

DyLight™ 650 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification follow by additional step of ultrafiltration is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for DyLight 650 fluorescent dye oligonucleotide labeling services.

Product Information

 

Product Name:

DyLight 650 Oligonucleotide Labeling

Category:

Fluorescent dye label, Alexa647, Cy5 Alternative Dye

Modification Code:

[DyLight650]

Exact Mass:

1066

Excitation (nm):

652

Emission (nm):

672

Extinction Coefficient, ε(λ):

250,000

Purification:

Dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.